A Method for Precise Analysis of Electrophoresis Pattern of Proteins from Samples Containing Large Quantity of Albumin

摘要

PURPOSE: A method for analysis of other proteins from an excessive albumin-containing sample using electrophoresis is provided, thereby easily and correctly analyzing other proteins adjacent to albumin as well as a trace amount of proteins. CONSTITUTION: The method for analysis of other proteins from an excessive albumin-containing sample using electrophoresis comprises the steps of: mixing the excessive albumin-containing sample with anti-albumin antibody resin; centrifuging the mixture; collecting the supernatant; and subjecting the supernatant to electrophoresis, wherein the excessive albumin-containing sample is serum, blood plasma or blood; the anti-albumin antibody resin contains 3 to 10mg of an anti-albumin antibody per 1ml of the resin; the anti-albumin antibody is a polyclonal antibody or a monoclonal antibody; and the anti-albumin antibody resin is prepared by non-covalently bonding the anti-albumin antibody with protein A resin or protein G resin or by covalently bonding the anti-albumin antibody with the resin.