Method for seedling production of Chinese foxglove Rehmannia glutinosa Lib. using bioreactor

摘要

PURPOSE: A method for culturing seeds of Rehmannia glutinosa using a bioreactor is provided, thereby effectively culturing high quality of Rehmannia glutinosa. CONSTITUTION: The method for culturing seeds of Rehmannia glutinosa using a bioreactor comprises the steps of: culturing stems of Rehmannia glutinosa in an MS(Murashige-Skoog) medium containing 1 mg/l of thidiazuron(TDZ) and 0.8% of agar for 4 weeks; culturing 90 of Rehmannia glutinosa shoots in 1.5 liter of an MS broth containing 1 mg/l of TDZ and 500 mg/l of MES£2-(N-morpholino)ethanesulfonic acid| in a bioreactor with aeration of 0.5 vvm for 3 weeks; culturing the shoots of Rehmannia glutinosa in a half concentration of MS medium containing 1.2% of agar and 0.3 to 0.4 mg/l of paclobutrazol in a culture bottle with 0.2 m of polyethylene membrane filter for 4 weeks to elongate and acclimate of Rehmannia glutinosa shoots; and treating the Rehmannia glutinosa shoots with an anti-GA like growth regulator.