METHOD OF SERUM DIAGNOSIS OF INFECTION HELICOBACTER PYLORI BY IMMUNOENZYME ANALYSIS

摘要

FIELD: medicine, gastroenterology. SUBSTANCE: method involves preparing total antigen from Helicobacter pylori, its sorption on solid phase and assay of antibodies raised to its in blood serum sample by immunoenzyme method. To enhance reliability of diagnosis before sorption on solid phase the total antigen is absorbed by immobilized complex "streptococcus protein G - human immunoglobulin" and detection of antibodies is carried out using conjugate "streptococcus protein G horse radish peroxidase". Invention provides the improvement of serum diagnosis method of Helicobacter pylori infection by immunoenzyme analysis, enhancement of its sensitivity and information content. Invention can be used for diagnosis of sicknesses associated with Helicobacter pylori. EFFECT: improved method of serum diagnosis. 3 cl, 2 tbl, 3 ex