FIELD: biotechnology. SUBSTANCE: invention relates to methods of preparing biologically active compounds enriched with selenium based on microalgae biomass. The proposed method of preparing selenium-enriched biomass of Spirulina platensis involves inoculate preparing by preliminary cells adaptation in culturing for 7-10 passages on agarized media with low content of sulfur (5-10% of norm) in the presence of 15-30 mg/l of sodium selenite and the use of the prepared inoculate for production carrying out in nutrient media with the same selenium content as in preparing inoculate and with content of sulfur 15-30 mg/l (5-10% of norm). Invention allows to obtain biomass of Spirulina platensis with different enrichment with selenium as component of biologically active compounds by regulation of process of its accumulation at high growth and biomass accumulation rate that provides high output by biomass yield. Invention can be used in phytobiotechnology, microbiology, medicine, algology, food industry and agriculture. EFFECT: improved method of biomass preparing. 4 tbl
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