RETROVIRAL VECTOR CONSTRUCTION SUBJECTING FOR PROMOTER CONVERSION, METHOD OF INCORPORATION OF HOMOLOGOUS OR HETEROLOGOUS NUCLEOTIDE SEQUENCE INTO HUMAN OR ANIMAL CELLS IN VITRO AND IN VIVO, RECOMBINANT RETROVIRAL PARTICLE, PHARMACEUTICAL COMPOSITION FOR GENETIC THERAPY

摘要

FIELD: molecular biology, genetic therapy, pharmacy. SUBSTANCE: invention relates to retroviral vector subjecting for promoter conversion comprising region 5'-long terminal repeat region from structure U3-R-U5; one or more sequences taken among encoding and non-encoding sequences; and region 3'-long terminal repeat region comprising the complete or partially deleted region U3. Indicated deleted region U3 is replaced for polylinker sequence after that regions R and U5 follow. Retroviral vector is subjected for promoter conversion and can be used as a carrier for gene transfer to carry out the directed genetic therapy. EFFECT: valuable properties of construction. 27 cl, 10 dwg, 3 ex