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METHOD FOR DIAGNOSING BRUCELLOSIS INFECTION IN HUMAN PATIENTS UNDER LABORATORY CONDITIONS

机译:实验室条件下诊断布鲁氏菌病感染的方法

摘要

FIELD: medicine. SUBSTANCE: method involves selectively condensing on magnoimmunosorbents, washing samples with their following boiling in water, genetically testing by applying polymerase chain reaction method. Blood serum is taken from patient and formed elements are destroyed by adding distilled water in 1:45- 1:50 proportion and stirring it. Selective condensation on magnoimmunosorbents is carried out after adding 10% brucellosis magnoimmunosorbents in the amount of 0.02 mg/l and incubating during 25-30 min. EFFECT: high reliability and sensitivity of method.
机译:领域:医学。物质:该方法涉及选择性浓缩在免疫免疫吸附剂上,在水煮沸后洗涤样品,通过应用聚合酶链反应法进行基因检测。从患者身上取血清,并通过以1:45-1:50的比例添加蒸馏水并搅拌来破坏形成的元素。在以0.02 mg / l的量添加10%布鲁氏菌病的magnoimmunosorbents并在25-30分钟内孵育后,对magnoimmunosorbents进行选择性浓缩。效果:方法可靠性高,灵敏度高。

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