POLYPEPTIDE ELICITING HEMOPOIETIC BIOLOGICAL ACTIVITY OF STEM CELL FACTOR (VARIANTS), DNA (VARIANTS), EXPRESSING VECTOR (VARIANTS), PHARMACEUTICAL COMPOSITION FOR HEMOPOIETIC THERAPY, METHOD FOR PREPARING POLYPEPTIDE AND METHOD FOR IT USING (VARIANTS)

摘要

FIELD: biotechnology, molecular biology, genetic engineering. SUBSTANCE: invention reports about novel polypeptides eliciting hemopoietic biological activity of factor of stem cells using genetic engineering methods. Polypeptides are expressed by the host cell carrying DNA-containing vector encoding one of variants of polypeptide. Method allows preparing recombinant polypeptide product as result of expression of DNA sequence. Polypeptides or recombinant polypeptide products are components of pharmaceutical composition used for hemopoietic therapy in mammals. Except for, they can be used for enhancement of effectiveness of genetic therapy, stimulation of early hematopoietic precursor-cells, enhancement of bone marrow grafting in transplantation, enhancement of degree of bone marrow recovery in aplasia treatment or myelosuppression of bone marrow caused by physical-chemical factors. Invention allows preparing polypeptides no occurring in the nature and having sufficiently duplicative amino acid sequences that can replace natural factors of the stem cell. EFFECT: improved preparing method, valuable medicinal properties of polypeptide. 82 cl, 68 dwg, 17 tbl, 20 ex