SINGLE-STRANDED PROTEIN, MODULATOR OF LUTEINIZING, FOLLICLE-STIMULATING, THYROID-STIMULATING AND CHORIONIC GONADOTROPHIC HORMONES (VARIANTS), NUCLEIC ACID MOLECULE (VARIANTS), VECTOR (VARIANTS), STRAIN OF TRANSFORMED CELL CHO (VARIANTS), METHOD FOR PRODUCING SINGLE-STRANDED PROTEIN (VARIANTS)

摘要

FIELD: biotechnology, endocrinology. SUBSTANCE: a single- stranded protein represents covalently bound - and - or - and -, or - and - subunits of luteinizing hormone, follicle-stimulating hormone, thyroid-stimulating hormone and chorionic gonadotrophic hormone. Amino acid sequences of subunits bind through linker or without its. The complete link of CTP is used as a linker consisting of from 112-118 to 145 amino acid of human chorionic gonadotrophic hormone subunit. A single-stranded protein is prepared by culturing cell of the strain CHO transformed with vector comprising nucleic acid that encodes a single-stranded protein. Invention provides preparing stable forms of hormones. Invention can be used in preparing single-stranded forms of glycoprotein hormones. EFFECT: improved preparing method. 24 cl, 4 dwg, 4 ex