Modified nucleic acid oligomer, useful for sequencing by hybridization, is substituted by redox agent to allow electrical detection of hybridization

摘要

Nucleic acid oligomer (I) modified by a redox-active substance (II) that is oxidizable and reducible selectively at a potential (phi) of 2 to -2 V, relative to the standard hydrogen electrode, is new. Independent claims are also included for the following: (a) a method for producing (I); (b) a modified conductive surface that has one or more types of (I) bound to it; (c) a method for producing surfaces of (b); and (d) a method for electrochemical detection of nucleic acid oligomer hybridization events, using the surface of (b).