Aav - vector - packaging plasmids to the helper virus - free production pseudo typical ized aav - particles by way of individual transfection

摘要

The invention relates to AAV vector packaging plasmids for producing (pseudotyped) AAV particles without helper viruses, by means of a single transfection. The AAV vector packaging plasmids for the (pseudotyped) AAV particles contain the following DNA sequences: (a) an AAV rep gene, (b) an AAV cap gene, (c) AAV expression vector DNA sequences and (d) all additional helper virus DNA sequences that are required for the formation of AAV particles. The AAV vector packaging plasmids for producing wtAAV particles are characterised in that they contain (a) the complete AAV genome and (b) all helper virus DNA sequences that are required for the formation of AAV particles. The invention also relates to the use of said AAV packaging plasmids for producing wtAAV particles or pseudotyped AAV particles, in particular for gene therapy or tumour therapy.