Apparatus for the detection of biochemical reactions has a substrate, with macro-pores, to immobilize catch molecules within them to make contact with the analytes and reactions measured by light transmission through the pores

摘要

Detecting biochemical reactions, a substrate (10) is used of silicon with macro-pores (11) passing through from at least at one side (10A,10B), with a diameter of 500 nm to 100 micro m. At least one catch molecule (20) is immobilized in each pore, in a specific location at the inner walls of at least part of the pores to give a biochemical reaction, using DNA, proteins and ligands. Analytes are introduced, to make contact with the catch molecules, using DNA, RNA, peptide nucleic acid (PNA), saccharide, peptide, proteins, cellular components, single cells, multi-cellular organisms and the like. A surface (10A) of the substrate is illuminated (40), and the light transmission through the pores is measured (50) at the other surface to show the reactions which have occurred within the pores.