New allosterically regulatable hairpin ribozyme, useful for identifying compounds that bind specific targets, has extra sequence between substrate binding and catalytic domains

摘要

Allosterically regulatable hairpin ribozyme (I) is new. Independent claims are also included for: (1) polynucleotide (II) comprises a hairpin ribozyme having a substrate-binding domain A and a domain B, where catalytic activity is based on contact between A and B, and also, between A and B, a domain C which connects A and B and comprises a nucleic acid that binds a target molecule; (2) polynucleotide (IIa) similar to (II) but where B is a catalytic domain and C is a nucleic acid at least partly complementary to a sequence that binds a target molecule; (3) biosensor that comprises (I), (II) or (IIa); (4) method for identifying compounds that bind to a target molecule; and (5) kit for method (4) comprising (I), (II) or (IIa) and a signal-generating ribozyme substrate.