首页>
外国专利>
thermostabile dna polymerase from bacillus pallidus
thermostabile dna polymerase from bacillus pallidus
展开▼
机译:苍白芽孢杆菌的热稳定DNA聚合酶
展开▼
页面导航
摘要
著录项
相似文献
摘要
A novel Type I DNA polymerase derived from Bacillus pallidus is provided. The gene encoding the polymerase has been cloned and expressed. Characterization of the enzyme revealed, in addition to DNA polymerase activity, 5'-3' exonuclease activity, reverse transcriptase activity, strand displacing activity and the ability to initiate polymerization at a nick in double-stranded DNA. In certain constructs the domain encoding the 5'-3' exonuclease activity is deleted to provide an enzyme which is particularly useful for Strand Displacement Amplification. Anti-Bacillus pallidus Polymerase I antibodies and methods of using the antibodies are also provided.
展开▼