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New peptide derivative heterobifunctional coupling agents containing aminooxy and maleimide groups, used for coupling aldehyde or ketone compound with thiol, especially for preparing diagnostic agents
New peptide derivative heterobifunctional coupling agents containing aminooxy and maleimide groups, used for coupling aldehyde or ketone compound with thiol, especially for preparing diagnostic agents
Heterobifunctional functional coupling agents (I), comprising peptide derivatives containing at least one 8-amino-3,6-dioxa-octanoic acid unit and aminooxy and maleimide functional groups, are new. Heterobifunctional functional coupling agents of formula (I), comprising peptide derivatives containing at least one 8-amino-3,6-dioxa-octanoic acid unit and aminooxy and maleimide functional groups, are new. W = NH-(CH2)2O(CH2)2-OCH2CO; A = (W')p-CO-(CH2)u-Mal (a), (W')p-CO-CH-((CH2)rNH-(W')p-CO-(CH2)u-Mal)2 (b) or (W')p-CO-CH(-(CH2)rNH-(W')p-CO-CH(-(CH2)r-NH-(W')p-CO-(CH2)u-Mal)2)-(CH2)q-NH-(W')p-CH(-(CH2)r-NH-(W')p-CO-(CH2)u-Mal)2 (c); D = group (a), (b), (c) or CO-(CH2)u-Mal (d); Mal = maleimide; Y = group terminated by CONH2 and inert with respect to the remainder of the molecule; W' = COCH2O-(CH2)2O(CH2)2-NH; n = 0-3; m, o = 0-3; p = 1-4; q = 0-3; r = 2-5; s = 0 or 1; t = 1-7, and u = 2-10. Independent claims are also included for: (1) new activated intermediates (II) comprising (I) and a molecule (II') having at least one surface aldehyde and/or ketone function before conjugation; (2) new activated intermediates (III) comprising (I) and 1-8 (preferably 1-4) molecule(s) (III') having at least one surface thiol function before conjugation; and (3) new conjugates (IV) comprising (I) bonded to (II') and to 1-8 molecule(s) (III').
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机译:包含具有至少一个8-氨基-3,6-二氧杂-辛酸单元和氨氧基和马来酰亚胺官能团的肽衍生物的异双功能官能偶联剂(I)是新的。式(I)的异双功能官能偶联剂是新的,其包含含有至少一个8-氨基-3,6-二氧杂-辛酸单元和氨氧基和马来酰亚胺官能团的肽衍生物。 W = NH-(CH2)2O(CH2)2-OCH2CO; A =(W')p-CO-(CH2)u-Mal(a),(W')p-CO-CH-((CH2)rNH-(W')p-CO-(CH2)u-Mal )2(b)或(W')p-CO-CH(-(CH2)rNH-(W')p-CO-CH(-(CH2)r-NH-(W')p-CO-(CH2 )u-Mal)2)-(CH2)q-NH-(W')p-CH(-(CH2)r-NH-(W')p-CO-(CH2)u-Mal)2(c) ; D =(a),(b),(c)组或CO-(CH2)u-Mal(d); Mal =马来酰亚胺; Y =由CONH2终止并且相对于分子其余部分呈惰性的基团; W'= COCH 2 O-(CH 2)2 O(CH 2)2 -NH; n = 0-3; m,o = 0-3; p = 1-4; q = 0-3; r = 2-5; s = 0或1; t = 1-7,而u = 2-10。还包括以下方面的独立权利要求:(1)新的活化的中间体(II),其包含(I)和在缀合之前具有至少一种表面醛和/或酮功能的分子(II'); (2)新的活化的中间体(III),其包含(I)和在缀合之前具有至少一个表面硫醇功能的1-8(优选1-4)个分子(III'); (3)新的缀合物(IV),其包含与(II')和1-8个分子(III')键合的(I)。
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