TRANSFORMANT FOR MEASURING DIOXINS, METHOD FOR DETECTING SAME BY USING SAME, METHOD FOR QUANTITATIVELY ANALYZING SAME AND METHOD FOR SCREENING SAME

摘要

PROBLEM TO BE SOLVED: To provide a transformant for measuring dioxins, capable of rapidly measuring large amounts of specimens which are required to be treated for surveying environmental pollution and capable of evaluating biological effects without causing inconvenience, to provide a method for detecting the dioxins by using the transformant, to provide a method for quantitatively analyzing the dioxins, and to provide a method for screening the dioxins.;SOLUTION: This transformant for measuring the dioxins constantly produces or induces an AhR and an Arnt, and produces a reporter protein through combination with a transcription control region of a complex containing the dioxins, the AhR, and the Arnt. The dioxins are detected by using the transformant. The dioxins are measured by GC-MS after the reporter protein is measured. Concentrations of the dioxins are measured based on an expression level of the reporter protein. Biological action levels of the dioxins are evaluated and screened by using the method for detecting the dioxins or the method for quantitatively analyzing the dioxins.;COPYRIGHT: (C)2004,JPO