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IMMOBILIZED CELL, METHOD FOR PRODUCING STRONGLY IMMOBILIZED CELL AND METHOD FOR PRODUCING CADAVERINE

机译:固定化细胞,强力固定化细胞的制备方法和卡维汀的制备方法

摘要

PROBLEM TO BE SOLVED: To provide a method for producing cadaverine, by which the cadaverine can stably industrially be produced with immobilized cells in high efficiency and in a high yield for a long period with the reduced consumption of energy without needing an organic solvent.;SOLUTION: Cells to whose surface layers a lysine decarboxylase is immobilized are produced so that the lysine decarboxylase contacts with an L-lysine salt in high efficiently, and then further entrapped and immobilized with κ-carrageenan gel. The immobilized cells are contacted with an L-lysine salt aqueous solution. The cadaverine can be produced in a high yield by using cells cultured also after entrapped and immobilized or by again adding vitamin B6 in the halfway of the reaction.;COPYRIGHT: (C)2005,JPO&NCIPI
机译:解决的问题:提供一种尸胺的生产方法,通过该方法可以在不使用有机溶剂的情况下以高效率和高收率长期稳定地生产固定化细胞的尸胺,同时减少能源消耗。解决方案:产生固定有赖氨酸脱羧酶表面层的细胞,使赖氨酸脱羧酶高效地与L-赖氨酸盐接触,然后进一步被κ-角叉菜胶包埋和固定。使固定的细胞与L-赖氨酸盐水溶液接触。通过使用包埋和固定后培养的细胞或在反应中途再次添加维生素B6,可以高产率地生产尸胺。COPYRIGHT:(C)2005,JPO&NCIPI

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