METHOD FOR INTRODUCING POLYNUCLEOTIDE INTO HOST CELL IN HIGH EFFICIENCY WITHOUT PERFORMING ACTIVATION CULTURE

摘要

PROBLEM TO BE SOLVED: To provide a transgenic method in which experiments can be carried out in a short time, having high efficiency.;SOLUTION: A polynucleotide is simply introduced into a host cell in high efficiency. The transgenic method comprises introducing the polynucleotide into the host cell by a method such as electroporation or lipofection and then carrying out culture of Escherichia coli in a selection medium without performing activation culture in an activation culture medium.;COPYRIGHT: (C)2004,JPO