Expression of the endogenous gene by non-homologous recombination with the cellular DNA of the vector construct

摘要

(57) SUMMARY The present invention, in general, to activate the expression of genes relating to or to be over-expressed genes by recombinant methods in insitu. The present invention is, in general, at a higher level than the level found in normal cells, to a method for expressing in a cell the endogenous gene. In one embodiment of the present invention, the expression of the endogenous gene is increased or activated after incorporation into cells by illegitimate recombination or non-homologous recombination of the regulatory sequences that activate gene expression. In another embodiment, the expression of the endogenous gene, selection for increased copy number of the amplifiable marker of one or more present in the vector that was built and installed simultaneously an amplifiable marker in one or more it is possible to further increase by the. The present invention, the target sequences are not required for integration, there is provided a method for performing identification and expression of genes can not be found in the current method. The present invention provides (in particular, cDNA molecules) the method of isolating cells expressing the transmembrane proteins such may be a transmembrane protein and heterogeneous methods of isolation of a nucleic acid molecule encoding a transmembrane protein . The present invention relates to a composition comprising a nucleic acid molecule genes, and gene products such that the gene, gene products, and nucleic acid molecules, isolated. These can be used in therapeutic and diagnostic applications different. Therefore, be made without the present invention uses a prior knowledge of the characteristics of the expression sequence of a gene, structure, or function, isolation and activation of endogenous genes, including genes associated with development and human disease possible.