FLUORESCENCE MICROSCOPE, MEASURING METHOD OF FLUORESCENT LIFETIME, AND MEASURING PROGRAM OF FLUORESCENT LIFETIME

摘要

PROBLEM TO BE SOLVED: To provide a fluorescence microscope for measuring a fluorescent lifetime by an inexpensive constitution.;SOLUTION: A semiconductor laser 1 irradiates laser light which is excitation light toward a sample 6 which is an observation object. A duty ratio adjuster 12 varies the ratio between the ON time and the OFF time of the semiconductor laser 1 by a command from a computer 10. A CCD 9 has a function for accumulating as long as a fixed time fluorescence radiated from the sample 6 and integrating the fluorescence. A plurality of integrated fluorescences are acquired from the CCD 9 by changing the ratio between the ON time and the OFF time of the semiconductor laser 1. Then, the computer 10 calculates the fluorescent lifetime by fitting a target function including the fluorescent lifetime as a parameter to a change curve of the integrated fluorescence relative to the ratio between the ON time and the OFF time of the semiconductor laser 11.;COPYRIGHT: (C)2004,JPO&NCIPI