PRETREATMENT AGENT, PRETREATMENT METHOD, MEASUREMENT METHOD BY PRETREATED SAMPLE, MEASURING KIT, AND DETERMINATION METHOD OF SAMPLE

摘要

PROBLEM TO BE SOLVED: To provide a rapid measurement method having a high detection rate for β-glucan in a blood-derived sample.;SOLUTION: (1) This pretreatment agent contains an alkali metal hydroxide as a main constituent. (2) This pretreatment method is characterized by mixing the pretreatment agent described in (1) with a sample and heating them. (3) This measurement method is used for measuring (1-3)-β-D-glucan in the sample by using a limulus reaction and for detecting variation of a substrate by pretreating the sample by the method (2), and by mixing and reacting the pretreated sample with a limulus reagent. (4) This measuring kit comprises (A) the pretreatment agent and (B) the limulus reagent. (5) This determination method of the sample is used for quantitatively measuring the (1-3)-β-D-glucan by the measurement method to determine that the sample is affected by an infectious disease when the substance exceeds a certain amount.;COPYRIGHT: (C)2004,JPO