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Production method of stabilization reaction mixture containing one or more enzymes of dried completely or partially, kits containing the same, and as well as the reaction mixture

机译:包含一种或多种完全或部分干燥的酶的稳定化反应混合物的生产方法,含有该酶的试剂盒以及反应混合物

摘要

An aqueous solution of a reaction mixture comprising at least one enzyme (a), (b) dry protection agent one invention, phosphate groups between the amine or a carboxyl group or (ii) carbonyl (i) at least a single vessel step of contacting, and an aqueous solution of a stabilizing mixture comprising, an inert polymer of at least one that can generate a net structure for preventing the at least one inhibitor inhibits the movement of (iii) dry reagent condensation reactions on how to include the. The invention further includes the step of removing some or all of the water contained in the aqueous solution is generated. Enzymatic reactions, for example, nucleic acid amplification, characterization of the execution sequencing and execution of hybridization tests, and the method is suitable for restriction analysis.
机译:反应混合物的水溶液,其包含至少一种酶(a),(b)一种干燥保护剂,一种在胺或羧基之间的磷酸基或(ii)羰基(i)至少一个接触的单一容器步骤以及稳定混合物的水溶液,该稳定混合物的水溶液包含至少一种惰性聚合物,该惰性聚合物可以生成用于防止至少一种抑制剂的网状结构,从而抑制(iii)干试剂缩合反应的移动,包括如何进行。本发明还包括除去产生在水溶液中的部分或全部水的步骤。酶促反应,例如核酸扩增,执行测序的表征和杂交测试的执行,该方法适用于限制性分析。

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