Determination of trace analytes by affinity capillary electrophoresis

摘要

(57) Abstract Quantitative method of detection of trace analysis ones in the sample is disclosed. The manner in the desirable example offers the immunoglobulin Fab'huragumento which the indicator is done with the fluorescent dye in the reactivity suruhuhidoriru basis; The sample and the said indicator Fab'huragumento which is the possibility of including analysis ones adjusting; The component of the blend which is obtained is concentrated with electric field; By capillarity tube electrophoresis, indicator analysis ones which were formed/the operation material complex is separated from all unreacted indicator operation material; Furthermore indicator analysis ones/the fact that the fluorescent signal of the operation material complex is detected is included. In addition as for this invention, it regards also the production manner of indicator Fab'huragumento, but as for this manner, disconnecting the immunoglobulin numerator, obtaining one F (ab') ₂ fragments; Disulfide join of the said F (ab') ₂ fragments is reduced, obtaining two Fab'huragumento where each at least has one free reactivity suruhuhidoriru basis; Furthermore, Fab'huragumento which at least has one free suruhuhidoriru basis, is mixed with the fluorescent dye which reacts with the free suruhuhidoriru basis, the fact that indicator Fab'huragumento is generated is included. It is desirable, before the last mixed process, disulfide join inside the chain it is formed by oxidation inside each Fab'huragumento, individual Fab'huragumento each one Fab'huragumento which has one reactivity suruhuhidoriru basis is produced with this. In addition as for this quantitative method of detection, compared to wide-ranging target analysis ones and use of each biological unique operation material which forms the complex include.