Estimation method of drug function using small hepatocytes rich colony, a process for their preparation, maturation Method for its liver tissue, the small hepatocytes rich colonies maturation

摘要

Method for preparing an implantable liver tissue according to the present invention, there is provided a process for its preparation and cell colonies suitable for that. Small hepatocytes rich cell colonies are composed of small hepatocytes about 70% of the total cell numbers, in particular, small hepatocytes rich cell colonies of the invention, made from the cells of about 30 to about 10 to , it is a cell colonies small hepatocytes number accounts for about 70% or more of the total number of cells. Process for the preparation of small hepatocytes containing high amount of such colonies are also disclosed. In addition, the action of the drug, and normal, especially liver function using a method of inducing the maturation of the liver tissue of small hepatocytes rich cell colonies of the present invention, a small hepatocytes rich cell colonies induced maturation How to estimate in vitro, effects associated also disclosed.