General labeling means of proteins using the wheat germ cell-free protein synthesis system

摘要

After changing the selector methionine methionine of cell-free protein synthesis for cell extracts, which had been obtained by completely removing the endosperm that utilizes a wheat germ cell-free protein synthesis system, mixed in the embryo bud extract from wheat germ extract , it was the manufacturing and production process of selenium methionine labeled protein which is characterized by using the protein synthesis reaction composition comprising a selector methionine instead of methionine, dialysis or under batch conditions, to carry out cell-free protein synthesis I provide the protein. Further provided is a protein that is produced and the production method of deuterium labeled protein using a similar approach.