Chinese hamster ovarian cell variant

摘要

PURPOSE: To obtain the variant strain useful as a host for producing a physiologically active substance (a glycoprotein, etc.) without any H-D antigenicity by mutating a Chinese hamster ovarian(CHO) cell and reducing the productivity of N-glycolylneuraminic acid. ;CONSTITUTION: This variant strain is obtained by mutating a CHO cell and selecting a strain having a reduced productivity of N-glycolylneuraminic acid (NeuGc). UV rays, X-rays, etc., are used as a physical mutagen and an alkylating agent, especially ethylmethanesulfonate is preferred as a chemical mutagen. The resultant 1A51 strain (FERM P-14066) obtained by the mutation has about 5/14 productivity of NeuGc and ≤1/5 hydroxylase activity of cytidine 5'-monophosphate-N-acetylneuraminic acid (CMP-NeuAc) as compared with those of CHO-K1 cell which is a parent strain.;COPYRIGHT: (C)1995,JPO