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Methods, compositions, and kits for mutation detection in mitochondrial DNA
Methods, compositions, and kits for mutation detection in mitochondrial DNA
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机译:线粒体DNA突变检测的方法,组合物和试剂盒
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摘要
Methods, compositions, and kits for detecting mutations in the entire human mitochondrial genome. A preferred method includes amplifying mtDNA from a biological sample by polymerase chain reaction of total DNA using a plurality of pre-selected primer pairs to generate overlapping amplicons; cleaving the amplicons using restriction enzymes to produce fragments suitable for analysis by denaturing high performance liquid chromatography (DHPLC); denaturing and re-annealing the amplicons; and fragment analysis by DHPLC to detect the presence or absence of heteroduplex molecules.
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