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Methods for detecting and adjusting replication of hepatitis b virus and for preparing mutated virus strains with altered replication efficiency
Methods for detecting and adjusting replication of hepatitis b virus and for preparing mutated virus strains with altered replication efficiency
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机译:检测和调节乙型肝炎病毒复制并制备复制效率改变的突变病毒株的方法
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摘要
The invention provides methods to assay and regulate the replication of hepatitis B virus (HBV), employing methods of generating HBV replication mutants. Based on the polymerase gene of HBV strains, various primers were designed, and by using restriction enzymes and polymerase chain reaction, fragments of the polymerase gene were substituted, and the replicative competency of the newly constructed recombinant polymerase gene was analyzed. This invention can be used to look for new functional domains in the polymerase and for designing anti-HBV replicating drugs and diagnostic reagents.
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