Oligonucleotide - based logic gates and molecular networks

摘要

A set of deoxyribozyme-based logic gates are capable of generating any Boolean function. The gates include basic NOT and AND gates, and the more complex XOR gate. These gates were constructed through modular design that combines molecular beacon stem-loops with hammerhead-type deoxyribozymes. The gates have oligonucleotides as both inputs and output, thereby communication between various computation elements in solution. The operation of these gates is conveniently connected to a fluorescent readout.