Method and system for the co-isolation of cognate DNA, RNA and protein sequences and method for screening co-isolates for defined activities

摘要

The present invention provides a method and a system for the co-isolation of cognate DNA, RNA and protein sequences, a method for screening co-isolates for defined activities, and the production of operons for simultaneous or coordinated expression of genes. Encapsulating solutions and microstructures in lipid vesicles or polymer capsules permits transcription/translation reactions amenable to techniques and instruments that utilize an aqueous environment. Microstructures containing binding sites for both mRNA and protein and encapsulated into a liposome or hollow polymer capsule, creating a reactosome. After transcription/translation, or other reactions, the reactosomes may be screened using aqueous methods. Once the desired reactosomes are isolated, the vesicles are disrupted, and the microspheres can be collected. Once transcription/translation reactions occur, the capsules may be frozen for storage or later use. Additionally, co-isolates may be screened for a desired activity before transcription/translation occurs. Operons may be produced in the capsules, separated, and isolated for later use.