Method for maintaining high-quality focus during high-throughput, microscopic digital montage imaging

摘要

Today's technology allows montage images of large areas to be captured, stored and displayed at the resolution limit of the microscope optics. The invention reduces the overall time to capture a microscope slide by reducing the overhead associated with refocusing the optics at each tile location. Using a macroscopic image of the region to be scanned, representative focus positions are selected based on a predefined set of image characteristics. Prior to montage scanning, these focus positions are placed under the microscope optics and a best-focus position determined. A surface is fit to the resulting three-dimensional data. The parameters that define this surface are feed into the scanning control component to allow high-quality focused images to be acquired throughout the scanning process, eliminating the required stop, refocus, acquire image processing steps used in traditional montage imaging systems.