Quantitative transient protein expression in plant tissue culture

摘要

The subject invention is related to a method for recombinantly and transiently producing a polypeptide in a plant tissue, which includes providing a plant tissue sample in a bioreactor; adding to the plant tissue sample, a sample of Agrobacterium containing a nucleotide sequence encoding the polypeptide on the T-DNA, co-culturing the plant tissue sample with the Agrobacterium so that the nucleotide sequence is transferred to the plant, allowing the plant tissue to transiently express the polypeptide, and then separating the polypeptide from the mixture.