Method for preparating an anemone raddeana extract and the pharmaceutical composition containing the same as well as use thereof

摘要

The present invention discloses a method for preparing Anemone Raddeana extract, comprising the following steps: a) pulverizing a rhizome, stem, leaf, flower, and fruit of Anemone Raddeana to obtain crude powders of Anemone Raddeana; b) extracting the crude powders of Anemone Raddeana with an ethanol solution, filtering, and recovering the ethanol; c) absorbing by a macroporous absorbing resin, removing pigments and saccharides, then eluting the resin until colorless with water or aqueous alkali solution, thus the fraction of saponin is retained in tile column; d) eluting with 5070% ethanol, recovering tile ethanol, adding gelatin, filtering precipitate, decoloring with decolor macroporous resin, eluting with 5070% ethanol, recovering the ethanol, concentrating and drying to obtain crude saponins of Anemone Raddeana. The present invention further relates to a method for extracting Raddeanin A. The method of the invention is convenient and suitable for industrial production. The Anemone Raddeana extract of the invention is formulated to pharmaceutical composition with pharmaceutically acceptable carriers. The pharmaceutical composition is used for treating malignant neoplasm diseases.