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Transcription-based assay for identification of post-translational modification and its application in proteomics
Transcription-based assay for identification of post-translational modification and its application in proteomics
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机译:基于转录的鉴定翻译后修饰的方法及其在蛋白质组学中的应用
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摘要
The present invention relates to a method for detecting the post-translational modification of a target protein by a post translational modifier polypeptide molecule; a method for screening a candidate protein for E3 ligase activity; a method of screening a test compound for the ability to regulate the post-translational modification of a target protein by a post-translational modifier polypeptide molecule; and a method for the large-scale detection of candidate target proteins of post-translational modification by a modifier polypeptide molecule. The present invention also relates to a kit for determining whether a test protein is post-translationally modified by a modifier polypeptide molecule; a kit for screening a test compound for the ability to regulate the post-translational modification of a target protein by a post-translational modifier peptide molecule, and another kit for determining whether a test protein is post-translationally modified by a modifier polypeptide molecule.
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