Strain of the methylotrophic yeasts producing recombinant homodimer of Human PDGF BB, DNA constructs and Methods for obtaining the strain

摘要

Metilotru00f3ficas recombinant yeast Strain producing a homodimer of Human PDGF BB, DNA constructs and Methods for obtaining the strain.The Invention relates to a recombinant Pichia pastoris Strain producing a homodimer of PDGF BB, comprising in their Genome at least a copy of the first Construction of DNA and at least one copy of a Second Construction of DNA, where such DNA constructs are Layer CES jointly lead the expression and release to the Environment of a homodimer.Where this Strain produces more than 200 mg / l of the dimer of PDGF BB.The Strain of the invention comprises: a) a First insertable DNA Sequence corresponding to a 5 'Regulatory sequence (Promoter) coupled to a Signal Sequence operablemente (b) (c) United operablemente to export a sequence that encodes the mature portion of the PDGF B Chain of being This sequence is a sequence of DNA base pairs that comprises 327 1226 from base to base 1552 of the coding sequence of PDGF.While the United operablemente d) sequence to a sequence of 3 'end of the transcript and a selection gene e) f) attached to a Second insertion sequence and Construction of DNA; The Second comprises: a) a First insertable DNA Sequence which corresponds to a region 5 'Regulatory operablemente (Promoter) coupled to a Signal sequence (b) United operablemente export(c) a sequence that encodes the mature portion of the b-chain of PDGF.This sequence is a sequence of DNA base pairs that comprises 327 from base to base 1552 1226 of the coding sequence of PDGF in the United operablemente Sequence d) a sequence of 3 'end of the transcript and linked to a Resistance Gene for Selection) Different Gene Selection the first Construction of DNA.