A method for Staining of harvested mammalian sperm Cells

摘要

A method for Staining of sperm cells collected from Mammals, whose purpose is to solve the problems with Conventional Methods in which, for example a Staining is not uniform, and the coloration time and the amount of dye attached to the DNA. Affect the viability of sperm fertilization resulting in lower ranks.The Method comprises the steps of: a) collecting semen of male Mammal; (b) incubating Sperm cells contained within the semen in a micro molar concentration of Hoechst 33342 Dye over 40; (c) establish the temperature at which the sperm cells in the concent Ration of Hoechst 33342 Dye are incubated for around 30 and around 40oC; or (d) adjustTime duration in which the sperm cells are incubated with the Hoechst 33342 Dye concentration between about 50 minutes and around 200 minutes; and (e) taking the DNA within the sperm cells with sufficient uniformity to allow the cell Sperm containing x chromosomes are distinct from Sperm cells containing chromosomesBased on the magnitude of fluorescence in a higher range of around 85%.