Method of obtaining support to be discarded in the imobilizau00e7ao enzyme invertase and the immobilization of the enzyme invertase

摘要

Method of obtaining support to be used in the immobilization of the enzyme invertase and immobilization of the enzyme invertase.Where the method of obtaining support to be used in the immobilization of the enzyme invertase comprises the steps of: extraction of humic substances existing in the soil; purification of substances hu00famiCAS with the extracted; elemental characterization of humic substances extracted and purified; the preparation of scaffolds using humic substances with the addition and activation of reagents on the basis of Mai.Nopropil silica to the solution.An integral part of the present invention immobilization of enzyme invertase which is obtained by the steps of: adding the enzyme invertase scaffolds prepared forming a mixture; maintain theThe mixture under mechanical stirring at room temperature for approximately 48 hours; filtering the mixture to a vacuum.Discarding the supernatant; submit to washing in acetate solution, the residue obtained (enzyme immobilized in the media) until the test results negative for invertase.