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Method for identifying animals or bovine carcasses, polinucleotu00ecdeo isolated, PCR product, pair of PCR primers, and kits for identifying animals or bovine carcasses

机译:鉴定动物或牛屠体的方法,分离的多聚核苷酸,PCR产物,PCR引物对和鉴定动物或牛屠体的试剂盒

摘要

Method of identifying animals or bovine carcasses, polinucleotu00ecdeo isolated, PCR product, pair of PCR primers, and kits for identifying animals or bovine carcasses. Provides methods and compositions to identify objectively if: (I) bovine animals having genetic potential to produce meat that is marmorizada or smooth.And (II) bovine carcasses whose meat is marmorizada or soft.The methods comprise extracting DNA from a sample obtained from a bovine animal or carcass, to test for the presence of a DNA comprising a sequ00b3u00eancia, referred to hereinafter as "marker gene".In the DNA sample. In one aspect, the genetic marker is a marker of mottling, and understand the sequ00b3u00eancia shown in SEQ ID No. 1.In another aspect, the genetic marker is a marker of softness, and understand the sequ00b3u00eancia shown in SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4 or combinations thereof.The compositions include primers that amplify markers of meat marmorizada or soft flesh present in genomes of bovine animal or carcass.And hybridization probes to detect markers of mottling or softness.
机译:鉴定动物或牛屠体的方法,分离的多聚核苷酸,PCR产物,PCR引物对和用于鉴定动物或牛屠体的试剂盒。提供方法和成分来客观鉴定以下情况:(I)具有产生潜伏或光滑肉的遗传潜力的牛,以及(II)潜伏或柔软的肉的牛cas体,该方法包括从获得的样品中提取DNA牛或or体,以检测包含seq的DNA(以下称为“标记基因”)在DNA样品中的存在。一方面,遗传标记是斑点的标记,并且理解SEQ ID No.1所示的seq u00b3 u00eancia。另一方面,遗传标记是柔软性的标记,并且理解seq u00b3 u00eancia SEQ ID No.2,SEQ ID No.3,SEQ ID No.4或其组合中所示。组成包括引物,该引物扩增牛动物或car体基因组中存在的or鱼或软肉的标记以及杂交探针以检测斑点或柔软的标志。

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