Su00ecntese a new paramagnetic amino acid derivative (EPM - 5) for use in the marking of different macromolecules and systems of biological interest quu00ecmico

摘要

"Su00ecntese a new paramagnetic amino acid derivative (EPM - 5) for use in the marking of different macromolecules and quu00ecmico biological systems of interest.The present invention relates to chemical synthesis and application as organic compound type paramagnetic spin marker, the product known as 2,2,5 acid.5 - tetramentilpirrolidina - 1 - oxy - 3 - [n - (fluorenilmetiloxicarbonil) Amin - 4 - carboxylic acid, abrevias peptide or of certain systems.Is possible, yet, the connection of the composed only by its radical POAC, with the removal of the protective Fmoc in base medium, organic, and, in the same way,When you need to extend the sequ00b3u00eancia peptide to the desired point.Because it contains a pyrrolidine ring type, its introduction in the middle of a peptide chain will cause different conformations of 244 - common amino acids.Therefore, a compound valuable to study the relationship structure biological activity of several peptides of importance.By the same synthesis process makes it possible to use Fmoc - POAC marking of the peptide angiotensin II (POAC ^ 7 ^ - Ali).