PROTEIN KINASE CANDIDA ALBICANS POSSESSING A NON-CYCLINE-DEPENDENT CAK ACTIVITY AND METHODS OF THEIR USES

摘要

1. An isolated Candida albicans protein kinase (CIV1), selected from protein kinase which comprises which comprises sequence of SEQ ID NO:2 and allelic variants thereof and determined by the following characteristics: lacks the motif GxGx(Y/F)GxV, in which G represents glycine, x represents any amino acid, Y/F represents either tyrosine or phenylalanine, V represents valine; possess a phosphorylation activity of non-cycline-dependent protein kinase. 2. Nucleic acid, encoding protein kinase according to claim 1. 3. Nucleic acid comprising sequence of SEQ ID NO:1. 4. Use of nucleic acid according to claim 3 for screening kDNA libraries from Candida albicans. 5. Use of nucleic acid comprising sequence of SEQ ID NO:1 or complementary nucleic acid for isolating protein kinase according to claim 1. 6. A recombinant vector for amplification nucleic acid according to any one of claims 2 or 3 obtained by embedding said nucleic acid into a suitable cloning vector. 7. A recombinant vector for expression nucleic acid according to any one of claims 2 or 3 obtained by embedding said nucleic acid into a suitable cloning vector. 8. A method for screening of fungicidal products, comprising a step of measuring kinase activity according to claim 1 in the presence of each product in which fungicidal properties are determined and selecting products inhibiting an effect to said activity.