METHOD AND KIT FOR THE QUANTITAVE MEASUREMENT OF SERUM LEVELS OF CLUSTERIN/APOLIPOPROTEIN J

摘要

his invention combines various technologies and renders the relative quantitative measurement of the of serum levels of Clusterin/Apolipoprotein J. It concerns a variation - adaptation of the ELISA methodology using two antibodies of which the second is conjugated chemically to the enzyme horseradish peroxidase (HRP, tracing enzyme) via which, by chromatic reaction, the serum levels of ApoJ are detected. Alternatively, it is possible that the second antibody does not bear the HRP enzyme conjugated to it, in which case, a third antibody is used against the Fc region of the species where the second antibody against ApoJ has been developed, which bears the HRP enzyme conjugated to it. The kit may be used for the prognosis in patients with diabetes type II or heart coronary disease, for the detection of vascular damage.