PLASMID FOR PRODUCTION OF CRM PROTEIN AND DIPHTHERIA TOXIN, MICROORGANISM TRANSFORMED WITH SUCH PLASMID AND METHOD FOR PRODUCTION OF CRM PROTEIN AND DIPHTHERIA TOXIN USING SUCH PLASMID AND MICROORGANISM

摘要

A method of producing diphtheria toxin or cross reactive material (CRM) protein that is cross reactive with diphtheria toxin, said method comprising: transforming a microorganism of the species Corynebacterium diphtheriae strain C7 with a plasmid containing a (a) gene encoding diphtheria toxin or CRM protein; (b) a Corynebacterium origin of replication; and (c) a selectable marker, and expressing said toxin or protein under conditions sufficient for expression of the gene by the microorganism. 2581 י" ב בתשרי התשס" ה - September 27, 2004