Production of chimeric polynucleotides useful for producing chimeric polypeptides comprises blunt-ending two polynucleotides and ligating the blunt ends

摘要

Production of chimeric polynucleotides by linking a double-stranded polynucleotide comprising a first gene or gene fragment at its 3' end to a double-stranded polynucleotide comprising a second gene or gene fragment at its 5' end comprises blunt-ending the two polynucleotides and ligating the blunt ends.