Flow cytometer analysis of cells and body fluids for fertility testing

摘要

A method of determining a fraction, number or concentration of mature versus immature sperm cells in a sample containing sperm cells and white blood cells, comprising: Labeling the white blood cells in the sample with a detectable label; For each cell in at least a portion of the sample, determining whether said cell is labeled or non-labeled and whether said cell is large or small; Determining a fraction, number or concentration of large and non-labeled cells and a fraction, number or concentration of small and non-labeled cells in the sample, or portion thereof; and Based on said fraction, number or concentration or large non- labeled cells to small non-labeled cells, determining a fraction number or concentration of mature versus immature sperm cells in the sample.