Methods for detection of target molecules and molecular interactions between nucleic acid tags attached to binding entities specific for the target molecules of interest

摘要

Methods of detecting target molecules are described together with the interactions between target molecules based on interactions between proximally-bound nucleic acid tags. Reagent kits for use in such methods are also provided. Figure 3 illustrates an exchange of sequence between the nucleic acid tags that results in a previously unknown detectable sequence. Examples of such detectable sequences include nucleic acid amplification primer binding sites or RNA polymerase binding sites, i.e., promoters that allow detection of the sequence by amplification. The sequences that promote the exchange of sequence are shown as triangles. One system that could be used is the phage lambda-based site-specific recombination system. In this example, the attL and attR recombination sequences recombine in the presence of recombinase to generate attP and attB.