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PRODUCTION AND USE OF GENE BANKS OF HUMAN ANTIBODIES (ßHUMAN-ANTIBODY LIBRARY)
PRODUCTION AND USE OF GENE BANKS OF HUMAN ANTIBODIES (ßHUMAN-ANTIBODY LIBRARY)
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机译:人类抗体基因库的生产和使用(ß人类抗体库)
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摘要
The gene library of human antibodies is taken from a mixture of human B-lymphocytes. Their mRNA are converted into cDNA using oligo-dT primers. The cDNA specific to antibodies are amplified by PCR with suitable oligonucleotide primer sequences. Using an expression of the antibody-specific cDNA in a bacterial expression vector, e.g. in E. coli, a human antibody library is produced with a wide range for screening with selected antigens in vitro.
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