PRODUCTION OF PICHIA PASTORIS YEAST AND SYSTEM FOR PURIFYING THE RECOMBINANT ALLERGEN OF OLEA EUROPAEA OLE E 1 FOR UTILIZATION IN THE DIAGNOSIS AND TREATMENT OF ALLERGIES

摘要

The invention relates to the production of Pichia pastoris yeast and a system for purifying the recombinant allergen of Olea europaea Ole e 1 for utilization in the diagnosis and treatment of allergies. The recombinant DNA coding for allergens Ole e 1 of olive tree pollen (Olea europaea), Syr v 1 from lilac pollen (Syringa vulgaris) and Lig v 1 of privet (Ligustrum vulgare) has been cloned and expressed. Said allergens have great structural similarity amongst them and with other pollen allergens of other Oleaceae. Recombinant proteins produced in the Pichia pastoris yeast are isolated with high yields, are correctly folded and exhibit immunological properties equivalent to those of natural allergens. Hence, they can be used in diagnosis protocol and immunotherapy.The invention relates to the production in Pichia pastoris yeast and a system for purifying the recombinant allergen of Olea europaea Ole e 1 for utilization in the diagnosis and treatment of allergies. The recombinant DNA coding for allergens Ole e 1 of olive tree pollen ( Olea europaea ), Syr v 1 from lilac pollen ( Syringa vulgaris ) and Lig v 1 of privet ( Ligustrum vulgare ) has been cloned and expressed. Such allergens have great structural similarity amongst them and with other pollen allergens of other Oleaceae. Recombinant proteins produced in the Pichia pastoris yeast are isolated with high yields, are correctly folded and exhibit immunological properties equivalent to those of natural allergens. Hence, they can be used in diagnosis protocol and immunotherapy.