Method capable of being automated for detection of prpless thanspgreater thanresless than/spgreater than and uses thereof

摘要

Process of detection automation of the prpres in a biological sample, as well as its applications. The detection method implements a solid support on which is immobilized the plasminogène.il essentially comprises the following steps: (a) a step of preparation of the biological sample during which the latter is incubated in a homogenization buffer which comprises a surfactant ionic or nonionic - ionic, a buffer must, a basic buffer, sucrose and a pbs buffer or in a capture buffer containing an ionic surfactant; this stage includes, optionally, the incubation with proteinase k, to a final concentration of between 1 and 8  g / ml, preferably at a final concentration of between 2 and 4  g / ml; (b) a step of capture of the prpres on said solid support, which is necessarily carried out in the presence of a capture buffer, such as defined -; (c) a denaturation step formed of prp-res fixed on said support comprising an incubation with a buffer for the denaturation comprising at least one chaotropic agent, at a temperature between room temperature and 100°C. and (d) a step of detecting prp-res denatured and fixed on said support with a specific antibody of the protein prp.