PCR METHOD AND APPLICATION BY TRANSNORMAL LOW THERMO-DENATURATION TEMPERATURE

摘要

The present invention relates to a method for polymerase chain reaction and application. It provides means for PCR amplification by transnormal low thermo­-denaturation temps which do not demand the use of DNA denaturant. The denaturation temperature of template can be used as follow: 93-98°C during first two or three cycles, and 60-87°C during continued cycles which transnormal low than normal denaturation temperature of 94-96°C. We discovered that this PCR method can be used widespread, and by means of the selective denaturation of template at transnormal temperature, we can control specificity of reaction, so this method can use for obviating unspecific PCR products, false negative result, false positive result comed from contamined products, and the method can used for distinguishing between gene DNA and cDNA.