MODEL ANIMALS NON-RESPONSIVE TO MYCOBACTERIA-ORIGIN LIPOPROTEIN/LIPOPEPTIDE

摘要

It is intended to provide TLR1 knockout mice specifically recognizing a mycobacteria-origin lipoprotein/lipopeptide, which are useful in clarifying the role of TLR1 in vivo, and a method of screening a promoter or an inhibitor for a response to a mycobacteria-origin lipoprotein/lipopeptide with the use of the same. The TLR1 knockout mice are constructed by isolating a TLR1 gene from a mouse gene library, substituting a part of the TLR1 gene containing the intracellular domain and the transmembrane domain by a neomycin tolerance gene, transferring an HSV-tk gene encoding thymidine kinase into the 3’-terminal side thereof, screening an ES cell clone double-resistant to G418 and ganciclovir, injecting the ES cell clone into the blastocyst of a C57BL/6 mouse and then getting the newborns from the reproductive system thereof.