Process for HLA Class 1 DNA typing using PCR amplification

摘要

An improved polymerase chain reaction (PCR) amplification technique is disclosed which is used to amplify HLA class I alleles, only a group of HLA class I alleles (such as HLA-B27) or only one specific allele, such as HLA-B*2703. The process uses a buffer containing about 10% DMSO and a chelating agent and is able to amplify genomic DNA. Oligonucleotide probes, having the same specificity as the primers, may be used to detect allele groups or individual alleles to determine whether a human has a high or low risk of being susceptible to arthritis.